Publication in EMBO Journal on metastatic exosomes in vivo

• Post by: OPL
• June 9, 2018
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With our collaborator Jacco van Rheenen we performed a study entitled “ TUMOR CELLS PHENOCOPY MIGRATORY BEHAVIOR IN VIVO VIA EXCHANGE OF EXTRACELLULAR VESICLES”, that was recently published in the EMBO Journal.

Synopsis:

Imaging microscopy in live animals combined with transcriptome-proteome analyses are used to characterize the function and content of extracellular vesicles (EVs) shed in vivo by mouse melanoma tumors with distinct metastatic potential. These data suggest that EVs promote cell migration by transferring clusters of RNA and proteins acting in the same physiological signaling networks.

• B16F1 and B16F10 melanoma cell clones with differential metastatic potencies functionally exchange EVs in vivo.
• The less metastatic B16F1 cells display increased invasive potential after uptake of EVs from the more aggressive B16F10 cells.
• EVs purified from B16F10 microenvironments contain more complex and distinct cargo networks of RNAs and proteins involved in cell migration than seen for B16F1.